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. 2008 Oct 17;283(42):28095–28105. doi: 10.1074/jbc.M708350200

FIGURE 6.

FIGURE 6.

NF-κB activity is increased by hypertonicity in mpkCCDcl4 cells. A, transfected cells expressing NF-κB-driven luciferase were challenged with either isotonic (Ctl, 300 mosmol/kg), hypertonic (NaCl, 500 mosmol/kg), or urea-supplemented hyperosmotic (urea, 500 mosmol/kg) medium for 12 h or were stimulated for 12 h with 100 μg/ml LPS in isotonic medium before measuring luciferase activity. Bars are the mean ± S.E. from four independent experiments. *, p < 0.05. B–D, cells were challenged with isotonic medium (Ctl, 300 mosmol/kg) or with either 100 ng/ml LPS (B), NaCl-supplemented hypertonic (400 or 500 mosmol/kg) (B) or urea-supplemented hyperosmotic (400 or 500 mosmol/kg) (C) medium for 2 or 24 h before RNA extraction. Real-time PCR was performed using primers specific for TNFα, MCP-1, or IκBα. Results are expressed relative to control values determined for each gene after 2 h of incubation in isotonic medium. Bars are the mean ± S.E. from five independent experiments. *, p < 0.05.