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. 2008 Oct 31;4(10):e1000193. doi: 10.1371/journal.ppat.1000193

Figure 3. Pretreatment with IFN-β primes DCs to respond more efficiently to virus infection.

Figure 3

(A,B) cDCs were pretreated with IFN-β (50 units/ml) for 3 hours. Following pretreatment, the IFN media was removed and cells were infected with PR8 virus (IFN+PR8) for 12 hours. Experiment was done in triplicate with error bars representing standard deviation between samples. All graphs have student t test p<0.05 between the IFN+PR8 condition and other conditions, with the exception of MIP-1β with p>0.05. (C,D) pDCs were pretreated with IFN-β (50 units/ml) for 3 hours. Following pretreatment, the IFN media was removed and cells were infected with PR8 virus (IFN+PR8) for 12 hours. Control pDCs were either infected only (PR8), pretreated with IFN only (IFN), or neither (NI). Mean of samples is depicted with error bars representing the standard deviation of each sample. All graphs have student t test p<0.05 between the IFN+PR8 condition and other conditions. (A,C) Copy number of mRNA expression values are depicted for the specific gene labeled. (B,D) Protein secretion amounts from multiplex ELISAs. Data are representative of at least 5 independent experiments.