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. 2008 Oct 27;3(10):e3518. doi: 10.1371/journal.pone.0003518

Figure 1. Subcellular localization of endogenous TRAF4 in fractionated extracts of MCF10A cells.

Figure 1

Protein fractionation from confluent normal/immortalized MCF10A cells was performed using the ProteoExtract Subcellular Proteome Extraction kit in order to separate cytoplasmic, membrane, and nuclear proteins. These fractions were analyzed by western blot using anti-E-cadherin, anti-9G8 and 2H1 anti-TRAF4 antibodies. TRAF4 is observed in all fractions whereas the membrane specific E-cadherin and nucleus specific 9G8 are only seen in membrane and nuclear fraction, as expected.