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Decreased eIF3f gene copy number in pancreatic cancer specimens. Laser captured microdissected pancreatic cancer tissues were collected and genomic DNAs were isolated. Relative eIF3f gene copy number was analyzed by quantitative real time PCR. The numbers are expressed as a ratio of eIF3f gene copy number over β-actin gene. We analyzed 10 normal samples and 12 pancreatic cancer tissues in triplicate.
