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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Mol Cell Neurosci. 2008 May 11;38(4):518–525. doi: 10.1016/j.mcn.2008.04.013

Figure 5. JNK inhibition prevents both c-jun phosphorylation and caspase-3 activation in cultured OSNs.

Figure 5

(a) In comparison to OSN cultures at time 0, OSNs cultured for 18 hours show a 3-fold increase in phosphorylation of c-jun as indicated by western blotting with an antibody to the phosphorylated form of c-jun (p-c-jun) when normalized to actin signal (2-fold when normalized to OMP signal). This increased c-jun phosphorylation is prevented when the same 18-hour culture is treated with JNK inhibitor VIII (JIVIII, 10 µM). (b) The levels of the kinase itself – both the 46kD (JNK1) and 54kD (JNK2/3) JNK family members – remain unchanged. (c) Caspase-3 activation is also evident after 18 hours in culture as indicated by an antibody to the cleaved fragment of caspase-3 and the downstream cleavage of PARP1. Both indicators of caspase activity are suppressed by treatment with JIVIII.