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Analysis of the internalization step of receptor-mediated endocytosis in ergΔ mutant cells. Internalization assays were performed at 24°C (open symbols) and 37°C (closed symbols) on erg2Δ (RH2897; A), erg6Δ (RH3622; B), and erg2Δerg6Δ (RH3616; C) mutant and wild-type cells (RH1800; A–C). Internalization of radiolabeled α-factor was expressed as a percentage by dividing internalized counts by the total cell-associated counts for each time point.
