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. 1999 Nov;10(11):3943–3957. doi: 10.1091/mbc.10.11.3943

Figure 5.

Figure 5

Analysis of α-factor degradation in ergΔ mutant cells. α-Factor degradation assays were performed at 37°C on wild-type (RH1800; A), erg2Δ (RH2897; B), and erg6Δ (RH3622; C) cells. At times indicated, samples were taken and diluted in pH1 buffer (1; internalized counts) or pH6 buffer (6; total cell-associated counts). Degradation of α-factor was assessed by extracting cell-associated radioactivity and separating degraded α-factor (d; indicating delivery to the vacuole) from intact α-factor (i) on TLC plates. d, degraded α-factor; i, intact α-factor; n′, time in minutes.