Table 1.
Strains and plasmids used in this study
| Strain/plasmid | Genotype/description | Source |
|---|---|---|
| Strains | ||
| RH448 | MATa his4 leu2 ura3 lys2 bar1 | Lab strain |
| RH1201 | MATα/MATa his4/his4 leu2/leu2 ura3/ura3 lys2/lys2 bar1/bar1 | Lab strain |
| RH1737 | MATa his4 leu2 ura3 bar1 sec18 | Lab strain |
| RH1800 (RH144-3D) | MATa his4 leu2 ura3 bar1 | Lab strain |
| RH1894 (RH296-10D) | MATα his4 ura3 erg6Δ | Lab strain |
| RH2622 (RH522-2C) | MATa his4 leu2 ura3 ade6? lys2? bar1 end11(erg2)-1 | Lab strain |
| RH2635 (RH144-3A) | MATα his4 leu2 ura3 bar1 | Lab strain |
| RH2180 (SF838-9D vps1-Δ2) | MATα his4 leu2 ura3 ade6 pep4 vps1-Δ2::LEU2 | T. Stevens |
| RH2894 [RH144-3A ERG2(END11)::URA3] | MATα his4 leu2 ura3 bar1 ERG2(END11)::URA3 | This study |
| RH2895 (RH2894 × RH2622) | MATα/MATa his4/his4 leu2/leu2 ura3/ura3 ADE6/ade6? LYS2/lys2? bar1/bar1 end11(erg2)-1/ERG2(END11)::URA3 | This study |
| RH2896 [RH1201 erg2(end11)-Δ1::URA3] | MATα/MATa his4/his4 leu2/leu2 ura3/ura3 lys2/lys2 bar1/bar1 erg2(end11-Δ1)::URA3/ERG2(END11) | This study |
| RH2897 | MATa his4 leu2 ura3 lys2 erg2(end11)-Δ1::URA3 bar1 | This study |
| RH2878 (RH407-1B) | MATa his3 leu2 ura3 ade2 lys2 bar1 | Lab strain |
| RH3610 (RH1894 × RH2897, 559) | MATα/MATa his4/his4 leu2/LEU2 ura3/ura3 lys2/LYS2 erg2(end11)-Δ1::URA3/ERG2(END11) erg6Δ/ERG6 bar1/bar1? | This study |
| RH3611 (RH559-8A) | MATα his4 ura3 erg6Δ erg2(end11)-Δ1::URA3 bar1? | This study |
| RH3612 (RH559-9B) | MATα his4 ura3 erg2(end11)-Δ1::URA3 erg6Δ bar1? | This study |
| RH3614 (RH3612 × RH2878, 562) | MATα/MATa his4/HIS4 leu2/LEU2 ura3/ura3 his3/HIS3 ade2/ADE2 lys2/LYS2 bar1/bar1? erg2(end11)-Δ1::URA3/ERG2(END11) erg6Δ/ERG6 | This study |
| RH3616 (RH562-8D) | MATa leu2 ura3 erg2(end11)-Δ1::URA3 erg6Δ bar1 | This study |
| RH3617 (RH562-1A) | MATa his (his3, his4, or his3 his4) ura3 erg2(end11)-Δ1::URA3 erg6Δ bar1 | This study |
| RH3622 | MATa his4 leu2 ura3 erg6Δ::LEU2 bar1 | This study |
| Plasmids | ||
| pBluescript KS− | ApR E. coli cloning vector | Stratagene |
| YCplac111 | ApR, LEU2-marked CEN/ARS E. coli/S. cerevisiae shuttle vector | Gietz and Sugino, 1988 |
| Ylplac211 | ApR, URA3-marked S. cerevisiae integration vector | Gietz and Sugino, 1988 |
| pEND11.2 | YCplac111 genomic library plasmid carrying ERG2(END11) on a 6.3-kb partial-Sau3A fragment | This study |
| pBKS-END11 | 2.9-kb BamHI fragment containing ERG2(END11) in pBKS | This study |
| Ylplac211-ERG2 | 2.9-kb BamHI fragment containing ERG2(END11) in Ylplac211 | This study |
| pend11-Δ1::URA3 | pBKS-END11 in which the BgIII–SphI fragment extending from nucleotides −110 to +495 relative to the start of ERG2(END11) was replaced by a 1.1-kb BamHI fragment containing URA3 | This study |
| pIU222 | YCp50 carrying a copy of ERG6 in which an internal 400bp XbaI–SaII fragment was replaced by the wild-type LEU2 gene | Gaber et al, 1989 |