Table 2.
Analysis of the sterol composition and the relative abundance of sterols within wild-type (RH448), erg2 (RH2897), erg6 (RH3622), and erg2erg6 (RH3616) cells
| Sterol | % sterol | Mass |
|---|---|---|
| WT | ||
| Zymosterol | 6.9 | 384 |
| Ergosterol | 77 | 396 |
| Fecosterol | 3 | 398 |
| A | 5.1 | 398 |
| Episterol | 3.2 | 398 |
| Lanosterol | 3.8 | 426 |
| erg2 | ||
| Squalene | 1.4 | 410 |
| Zymosterol | 5 | 384 |
| Ergosta-5,8,22-trienol | 12.7 | 396 |
| Ergosta-7,22-dienol | 4.3 | 398 |
| Fecosterol | 33.2 | 398 |
| Ergosta-8-enol | 35.4 | 400 |
| B | 1.6 | 396 |
| 4-m-Cholesta-8,24-dienol | 2.5 | 398 |
| Lanosterol | 2.9 | 426 |
| 4,4-dm-Cholesta-8,24-dienol | 1.1 | 412 |
| erg6 | ||
| Squalene | 1.3 | 410 |
| Cholesta-5,8,24-trienol | 2.8 | 382 |
| Zymosterol | 39.4 | 384 |
| Cholesta-5,7,24-trienol | 32.2 | 382 |
| Cholesta-7,24-dienol | 7.3 | 384 |
| Cholesta-5,7,22,24-tetraenol | 7.9 | 380 |
| C | 2.7 | 398 |
| Lanosterol | 3.1 | 426 |
| 4,4-dm-Cholesta-8,24-dienol | 2.3 | 412 |
| erg2erg6 | ||
| Squalene | 2.7 | 410 |
| Zymosterol | 85.6 | 384 |
| D | 3.7 | 382 |
| E | 1.5 | 398 |
| Lanosterol | 3.3 | 426 |
| 4,4-dm-Cholesta-8,24-dienol | 2.1 | 412 |
After growth at 24°C to midlog phase, cells were incubated for 30 min at 37°C. Total sterols were isolated from whole cells and subjected to GLC and GLC-MS to identify individual sterols and to determine the abundance of each sterol within a strain. WT, wild type; 4,4-dm-cholesta-8,24-dienol, 4,4-dimethyl-cholesta-8,24-dienol; 4-m-cholesta-8,24-dienol, 4-methyl-cholesta-8,24-dienol; A–E, identification of sterols uncertain.