Figure 4.

Apigenin and luteolin oppose IFN-γ-induced phosphorylation of STAT1 in N9 microglial cells. N9 murine microglial cells were seeded in 24-well tissue-culture plates (1 × 10⁵/well) and treated with IFN-γ (100 U/mL) in the presence or absence of apigenin and luteolin (25 μM) or treated with vehicle (1% DMSO; control) for 30 mins. Cell lysates were prepared from these cells and subjected to western immunoblotting using anti-phospho-STAT1 (Ser727) and anti-total STAT1 antibody as indicated. For B, data are represented as mean ratios of phospho-Stat1(Ser727) to total Stat1 (+/- SD) from densitometric analyses. Results are representative of three independent experiments. (**p < 0.01).