Figure 1.

Experimental design for BOTL-5 micro array hybridizations. Common reference experimental design showing microarray hybridizations for six BTB-infected animals and six control non-infected animals across the 12-hour bovine tuberculin stimulation time course. In all cases, the test sample was labelled with Cy3 fluorescent dye (base of the arrow) and the common reference RNA (CRR) pool was labelled with Cy5 dye (arrow head). The CRR pool consisted of equal amounts of total RNA from the BTB-infected and control animal groups at T0 (0 h pre-stimulation) assembled as described previously [24].