Figure 4.
Effects of drugs on hGM-CSF-dependent prevention of depletion induced apoptosis in BA/F3 cells. (A, C, and D) Effects of drugs on DNA fragmentation (A), caspase-3 activity (C), or mitochondria membrane potential (D) were examined. BA/F-wild (A, C, and D), -544 (A), and -Fall (A, C, and D) cells were depleted of mIL-3 and then treated with the indicated drugs. Cells were cultured 24 h, and DNA fragmentation, caspase-3 activity, or mitochondria membrane potential was examined by TUNEL analysis, enzyme assay using fluorescence-conjugated peptide substrate, by using DiOC6(3) as a membrane potential indicator, as described in MATERIALS AND METHODS. (B) Effect of various doses of genistein for anti-DNA fragmentation of hGM-CSF in the presence of PD98059. BA/F-wild cells were treated with various concentrations of genistein in the presence of PD98059 (100 μM) 15 min before stimulation by hGM-CSF, and DNA fragmentation was analyzed by TUNEL analysis after 24 h of culture.