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Kinetic Parameters for WT and Mutant PurEs.
| kcat(s-1)
N5-CAIR→CAIR |
kcat(s-1)
CAIR→N5-CAIR |
Km CAIR
(μM) |
Kd CAIR
(μM) |
Kd NO2-
AIR (nM) |
|
|---|---|---|---|---|---|
| WT | 15.5 ± 0.9 | 15.6 ± 0.2 | 22.2 ± 4.6 | ND | 86 ± 31 |
| H45N | < 0.0003 | < 0.00008 | NDa | 20.9 ± 1.9 | ND |
| H45Q | 0.0021 ± 0.0003 | 0.0012 ± 0.0004 | ND | 16.3 ± 2.6 | ND |
| H45W | < 0.0003 | 0.0004 ± 0.0002 | ND | ND | ND |
ND = not determined
