Figure 1.

Schematic illustration showing wild-type and mutant HRP-tyrosinase chimeras. The top line shows the components used for constructions as follows: hGH, human growth hormone signal; HRP, enzimatically active domain of HRP; P-Sel, the transmembrane domain of P-selectin; and Tyrosinase, the cytoplasmic domain of human tyrosinase. Boxes show the sequence boundaries of individual components. Sequences outside boxes show the components added during construction. The middle line shows the full amino acid sequence of the cytoplasmic tail of tyrosinase as used in wild-type HRP-tyrosinase. The bottom line shows the sequence of the cytoplasmic domain of tyrosinase with di-alanine substitution of di-leucine; HRP-tyrosinase/AA. Amino acid numbers from human tyrosinase are shown.