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Targeting of CD4-C2AB to SLMV in PC12 cells. PC12 cells expressing wild-type CD4-C2AB were fed with 100 ng/ml 125I-Q4120 in the presence (○) or absence (●) of 10 μg/ml BFA and fractionated on 5–25% glycerol gradients to isolate SLMV. The efficiency of SLMV targeting is expressed as the amount of radioactivity in each fraction across the gradient normalized to that in the homogenate.
