Figure 3.

Two mammalian Golgi proteins are targeted to the Golgi region of mosquito cells in the presence and absence of cholesterol. Control (A and C) or cholesterol-depleted C6/36 cells (B and D) were transfected with SFV RNA encoding either ManII (A and B) or β1,4GT (C and D), and fixed 16 h later. After permeabilization, cells were labeled with rabbit polyclonal sera specific for ManII or β1,4GT and Texas red-conjugated goat-anti-rabbit IgG. Both ManII and β1,4GT are targeted to punctate structures similar to those labeled by the Golgi marker, C₅-DMB-cer (Figure 2). Qualitatively, there is no difference in targeting of either transfected marker in the presence or absence of cellular cholesterol. Bar, 10 μm.