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. 1997 Nov;8(11):2111–2118. doi: 10.1091/mbc.8.11.2111

Figure 5.

Figure 5

The membrane anchor of β1,4GT can target a reporter protein to the Golgi region in C6/36 cells. Control (A and C) or cholesterol-depleted C6/36 cells (B and D) were transfected with SFV RNA encoding Iip31 (A and B) or a chimeric protein (C and D) with the membrane anchor of β1,4GT and the lumenal domain of Iip31 (1–47GT/Iip31), and fixed 16 h posttransfection. After permeabilization, cells were labeled with anti-Iip31 and Texas red-conjugated secondary antibody. The membrane anchor of β1,4GT is sufficient to redirect Iip31 from an endosomal/plasma membrane distribution (A and B) to a Golgi region distribution both in the presence (C) and absence (D) of cholesterol. Bar, 10 μm.