Figure 6.

Student photographs of Hoescht-stained cells to study the effects of anchorage dependence on apoptosis. Anchorage-dependent mouse L929 cells were cultured in 24-well dishes with or without paraffin coating to prevent attachment (D and A, respectively). To determine whether paraffin directly induces apoptosis, anchorage-independent HL-60 cells were cultured in the same way (E and B, respectively). Additionally, L929 cells were exposed to VP-16, a known inducer of apoptosis (F), or medium alone (C) without paraffin. In all cases, chromatin condensation was used to indicate apoptosis. Interestingly, no chromatin condensation was evident in L929 cells under any condition even following exposure to VP-16. Original magnification, 400×.