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. 1997 Nov;8(11):2145–2155. doi: 10.1091/mbc.8.11.2145

Figure 4.

Figure 4

Examination of G strand length by DMS cleavage. (A) lane 1, G-cleavage pattern of mature macronuclear DNA. Lanes 2-5, anlagen DNA isolated from cells treated with 0, 10, 20, or 40 μg/ml aphidicolin. (B) lane 1, G-cleavage pattern of mature macronuclear DNA; lane 2, anlagen DNA from untreated cells; lane 3, anlagen DNA from fivefold concentrated cells; lane 4, anlagen DNA from concentrated cells treated with 0.4% DMSO; lane 5, anlagen DNA from concentrated cells treated with 40 μg/ml aphidicolin. (C) Enlargement of lanes 4 and 5 from panel A showing the G-cleavage pattern extending beyond G95–98. All anlagen DNA was isolated at 60 h of development. The positions of the guanines relative to the 3′ terminus are marked at the side of each gel.