Figure 5.
Detection of long C-strands by Southern hybridization (T4G4)8. Lane 1, DNA from mature macronuclei; lanes 2–4, anlagen DNA isolated from control cells at 60 h of development; lane 2, DNA from untreated cells; lane 3, DNA from fivefold concentrated cells; lane 4, DNA from concentrated cells treated with 0.4% DMSO; lane 5, anlagen DNA isolated from aphidicolin-treated cells at 60 h of development; lane 6, anlagen DNA isolated at 44 h of development. The position of 1 kb and 2 kb markers are shown to the right.