Table 2.

Cell growth inhibition values (IC₅₀) expressed in log of molar concentrations for Doxaz alkyl carbamates (2 and 3) and alkyl PABC-Doxaz compounds (4 and 5) compared with Dox for 24 h drug treatment. Cancer cells are defined in the legends to Table 1 and Figure 3. Growth inhibition of cardiomyocytes (H9c2(2-1)) is a measure of cardiotoxicity, and growth inhibition of Vero cells (green monkey kidney cells) is a second measure of toxicity to normal cells. Viability of cells was assayed with crystal violet except the viability of Vero cells which was assayed with MTT.

Drug / Cell Line	Dox	2 Doxaz Butyl Carbamate	4 Butyl PABC Doxaz	3 Doxaz Pentyl Carbamate	5 Pentyl PABC Doxaz
MCF-7	−7.9 ± 0.04	−6.1 ± 0.04	−4.1 ± 0.3	−6.1 ± 0.03	−5.6 ± 0.3
MCF-7/Adr	−5.6 ± 0.1	−6.2 ± 0.3	−5.9 ± 0.06	−6.0 ± 0.09	−6.5 ± 0.06
DU-145	−7.5 ± 0.08	−6.4 ± 0.02	−6.3 ± 0.3	−6.0 ± 0.04	−6.7 ± 0.07
SHP-77	−6.9 ± 0.05	−6.6 ± 0.3	−7.1 ± 0.1	−6.3 ± 0.3	−7.3 ± 0.1
HepG2	−7.5 ± 0.1	−6.7 ± 0.3	−7.7 ± 0.03	−6.5 ± 0.3	−7.3 ± 0.2
SK-HEP-1	−7.3 ± 0.2	−6.4 ± 0.2	−5.8 ± 0.04	−6.7 ± 0.1	−6.0 ± 0.06
H9c2(2-1)	−7.7 ± 0.3	−5.8 ± 0.2	−7.2 ± 0.2	−6.6 ± 0.1	−6.2 ± 0.2
Vero	−6.3 ± 0.08	−6.3 ± 0.1	−6.1 ± 0.08	−5.3 ± 0.01	−6.0 ± 0.04