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. Author manuscript; available in PMC: 2008 Oct 17.
Published in final edited form as: Gastroenterology. 2006 Jun;130(7):2179–2188. doi: 10.1053/j.gastro.2006.03.024

Figure 7.

Figure 7

(A) Cells were labeled with JC-1, a mitochondrial membrane potential marker, and fluorescent properties were analyzed by flow cytometry after 0, 3, 6, 10, 16, and 24 hours of exposure to anti-Fas antibody (100 ng/mL) and actinomycin D (50 ng/mL). The percentage of apoptotic cells was significantly lower in the case of Hint2-silenced expression (42.4% ± 4.2% vs 60.9% ± 3.9% at 16 hours and 62.5% ± 8.6% vs 85.8% ± 3.7% at 24 hours; *P < .05). (B) Less cleaved caspase-3 was observed in HepG2 cells with silenced expression of Hint2 in response to anti-Fas antibody (100 ng/mL) and actinomycin D (50 ng/mL) for 16 hours compared with the other cell lines.