Figure 8.
Hint2 protein expression and tumor burden. (A) Effect of Hint2 overexpression on tumor size in SCID mice after subcutaneous injection of 3 × 106HepG2 cells stably transfected with pControl vector or HINT2cDNA (0.32 ± 0.13 g vs 0.85 ± 0.35g; *P < .05; n = 5 in each group). (B) Expression of cleaved caspase-9, PARP, and Hint2 was assessed by immunoblot analysis in the tumors. The level of expression of these proteins was significantly different between the Hint2 tumors and the pControl tumors (1.79 ± 0.07 vs 0.65 ± 0.06, 0.84 ± 0.09 vs 1.38 ± 1.1, and 3.62 ± 0.08 vs 0.54 ± 0.5, respectively; *P < .05).