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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Cell Metab. 2008 Aug;8(2):132–145. doi: 10.1016/j.cmet.2008.07.001

Figure 5.

Figure 5

Effect (mean ± SEM) of T cells on RANKL/OPG mRNA expression by BM SCs and SC osteoclastogenic activity. A mRNA levels of RANKL and OPG and RANKL/OPG mRNA ratio in SCs from nude mice. *= p<0.05 as compared to the corresponding vehicle group. **= p<0.05 as compared to the corresponding WT and T cell reconstituted groups. B mRNA levels of RANKL and OPG and RANKL/OPG mRNA ratio in SCs from WT mice depleted of T cells in vitro. *= p<0.05 as compared to the corresponding vehicle group. **= p<0.05 as compared to the corresponding WT and T cell reconstituted groups. C BMMs and T cells from WT mice were cocultured with SCs from nude and control mice for 1 week in the presence of PTH. Cocultures were stained for TRAP and OCs counted. *= p<0.05 compared to vehicle. **= p<0.05 compared to vehicle and to the corresponding PTH treated groups with SCs from WT and T cell reconstituted nude mice. D BMMs and T cells from WT mice were cocultured for 1 week in the presence of PTH with SCs from either WT BM depleted of T cells in vitro or control BM. *= p<0.05 compared to vehicle. **= p<0.05 compared to vehicle and to PTH treated groups with SCs from whole and reconstituted BM.