Table 2.
Primers and targeted genes in real time PCR
| Systematic Name | Gene symbol | Gene Title | Amplicon Size (bp) | Sequences (5’ – 3’) | |
|---|---|---|---|---|---|
| 18 srRNA | 149 | Forward: | GGTCATAAGCTTGCGTTGAT | ||
| Reverse: | TCAAGTTCGACCGTCTTCTC | ||||
| 1367568_a_at | Mgp | matrix Gla protein | 127 | Forward: | GCCTACAACCGCTACTTCAG |
| Reverse: | CGCACACGAATCTGTGTATC | ||||
| 1383047_at | Gas6 | Growth arrest specific 6 | 129 | Forward: | GTACATAGTGGGACTGATAC |
| Reverse: | TTCTTCAGATAGTCCCGCTC | ||||
| 1374529_at | Thbs1 | Thrombospondin 1 | 122 | Forward: | CTTAAAACTACTGTCGTGTC |
| Reverse: | ACCAGAACTTTGAGGAGTTG | ||||
| 1375961_at | Frzb | Frizzled-related protein | 127 | Forward: | ATTTGGCTCGCTGTATTGAC |
| Reverse: | GTGCACATTTCACTGTCCTG | ||||
| 1392915_at | Col11a1 | Procollagen type IX alpha 1 | 156 | Forward: | GCCAATCCATTTTATGCCAC |
| Reverse: | ATGAGCCCTGTTGCCATCTC | ||||
| 1370944_at | Col10a1 | Procollagen type X alpha 1 | 141 | Forward: | GAAACAGGTGTCTGACTTAC |
| Reverse: | TACTTCCAGTGGAATAGAAG | ||||
| 1367562_at | Sparc | Secreted acidic cysteine rich glycoprotein | 147 | Forward: | TGGGCTACAGGAAAGTGAGA |
| Reverse: | TGGCTTGGAAGTTTCTCTTG | ||||
| 1372299_at | Cdkn1c | Cyclin-dependent kinase inhibitor 1C | 125 | Forward: | ACCAGCTTCAGATTACCCAC |
| Reverse: | TCTAATAATGTGGAGGACAC | ||||
| 1371245_a_at | LOC689064 | hemoglobin beta-2 subunit | 108 | Forward: | CCTTTCCTGCTTGTCTATGC |
| Reverse: | TTTATTAGATAGAAGCTAGC | ||||
| 1387854_at | Col1a2 | Procollagen type I alpha 2 | 136 | Forward: | GAAGACTCGATCCTCCATAG |
| Reverse: | ATGCTGAATCTAGAGAGGAG | ||||
| 1381925_x_at | LOC497729 | hypothetical gene supported by NM_172157 | 125 | Forward: | GAAGAAACACAACTTCAAAG |
| Reverse: | GAAAAGGAGGAATTTACATC | ||||
| 1367631_at | CTGF | Connective tissue growth factor | 154 | Forward: | GACGTTTGTGCCTATTGTTC |
| Reverse: | CAGACAGTCACTCAGGTTAC | ||||
| 1367668_a_at | Scd2 | Stearoyl-coenzyme A desaturase 2 | 131 | Forward: | GCACATTTTATAGTAACCG |
| Reverse: | TTCCATAGCAATGAGTATAC | ||||
| 1374870_at | Col27a1 | Procollagen type XXVII alpha 1 | 126 | Forward: | AACCCTTCCCTGTATACTGC |
| Reverse: | AAGGAGACAGCGCAAGACAC | ||||
| 1392965_a_at | Smoc2 | Sparc-related modular calcium binding 2 | 124 | Forward: | AAACTGTTCATGGTCCCCAG |
| Reverse: | AGCGTTGATGTGAGCTGGTC | ||||
| 1373463_at | Col5a2 | Procollagen type V alpha 2 | 109 | Forward: | CCATCAATGACCAACACAAG |
| Reverse: | GGTCAGGCACTTCAGATCAT | ||||
| 1371226_at | Col2a1 | Procollagen type II alpha 1 | 104 | Forward: | CCGGACTGTGAGGTTAGGAT |
| Reverse: | AACCCAAAGGACCCAAATAC | ||||