Table 4.
Top 30 Genes in Reserve Zone (RZ) by highest expression
| 7 days | 11 days | 16 days | |||||
|---|---|---|---|---|---|---|---|
| Gene Title | Gene Symbol | MC | PCR | MC | PCR | MC | PCR |
| procollagen, type II, alpha 1 | Col2a1 | 11250 | 15076 | 4313 | 916.5 | 6432 | 594.3 |
| high mobility group box 1 | Hmgb1 | 2951 | 3613 | 4480 | |||
| procollagen, type IX, alpha 3 | Col9a3 | 4269 | 3031 | 4477 | |||
| NADH dehydrogenase subunit 3 | ND3 | 3689 | 2909 | 3270 | |||
| Similar to RIKEN cDNA 1110017I16 | RGD1308977 | 3629 | 757.1 | 884.7 | |||
| Microtubule-associated protein 7 | Mtap7 | 2038 | 2110 | 3236 | |||
| secreted acidic cysteine rich glycoprotein | Sparc | 3225 | 13.00 | 619.7 | 1.53 | 1071 | −22.09 |
| Similar to hypothetical protein FLJ32743 | RGD1306734 | 1326 | 1735 | 3007 | |||
| ribosomal protein L35a | Rpl35a | 2131 | 2127 | 2708 | |||
| Plexin B2 | Plxnb2 | 1972 | 2693 | 2219 | |||
| Catenin (cadherin associated protein), beta 1 | Ctnnb1 | 2399 | 2653 | 2418 | |||
| reticulocalbin 1 (predicted) | Rcn1 | 2498 | 1974 | 2566 | |||
| Hyaluronan and proteoglycan link protein 1 | Hapln1 | 1164 | 991.7 | 2516 | |||
| procollagen, type XI, alpha 1 | Col11a1 | 2506 | 93.70 | 1072 | 66.26 | 1686 | 48.84 |
| Carnitine palmitoyltransferase 1a, liver | Cpt1a | 2307 | 1502 | 1529 | |||
| gap junction membrane channel protein alpha 1 | Gja1 | 1178 | 2157 | 1114 | |||
| similar to Hemoglobin beta-2 subunit | LOC689064 | 935.8 | 28.05 | 2119 | 21.11 | 154 | 152.70 |
| procollagen, type I, alpha 2 | Col1a2 | 2019 | 272.48 | 1822 | 13 | 1207 | 109.50 |
| procollagen, type IX, alpha 1 | Col9a1 | 1969 | 867.1 | 1008 | |||
| similar to retinoblastoma binding protein 4 | LOC685491 | 1490 | 1950 | 1639 | |||
| eukaryotic translation elongation factor 1 alpha 1 | Eef1a1 | 1907 | 1140 | 738.5 | |||
| SPARC related modular calcium binding 2 | Smoc2 | 1901 | 67.18 | 107.1 | 15.30 | 38.31 | 13.78 |
| carboxypeptidase E | Cpe | 1879 | 691.9 | 534.8 | |||
| ribosomal protein S6 | Rps6 | 1858 | 772.9 | 567.6 | |||
| ribosomal protein L37 | Rpl37 | 1759 | 803.5 | 578.2 | |||
| hypothetical gene supported by NM_172157 | LOC497729 | 670.6 | 10.93 | 1759 | 4.96 | 962.8 | 21.48 |
| Calpastatin | Cast | 789 | 942.5 | 1756 | |||
| matrix Gla protein | Mgp | 1742 | 129.34 | 162.6 | 36.33 | 82.63 | 3.04 |
| similar to DnaJ homolog subfamily B member 6 | LOC686213 | 676 | 1699 | 1473 | |||
| ribosomal protein L13A | Rpl13a | 1612 | 656.3 | 428.9 | |||
| Correlationi coefficient R | 0.9720 | 0.8228 | 0.9152 | ||||
Note: Among the top 30 genes in RZ by highest expression in microarray (MC) analysis, eight selected representative genes were confirmed by real time PCR (PCR) with high correlation coefficient (R > 0.8). The comparative threshold (ΔΔCT) method was utilized for relative quantitative analysis of gene expression in real time PCR. The threshoyld cycle (CT) was normalized to the reference gene and the numbers represent relative quantitation for gene expression level..