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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2008 May 23;49(9):4026–4035. doi: 10.1167/iovs.08-1807

FIGURE 2.

FIGURE 2

Wild-type and a mutant form of HSV-1(KOS) can enter into HCjE cells. (A) Analysis of wild-type HSV-1 entry into HCjE cells. HCjE or control CHO-K1 cells were plated in 96-well culture dishes and inoculated by serial dilutions of β-galactosidase– expressing recombinant HSV-1(KOS) gL86 virus at the plaque-forming unit (PFU)/cell indicated (a). The soluble substrate, ONPG, was added to cells at 6 hours after infection, and the enzymatic activity was measured with a spectrophotometer (a). Similar as-says were repeated with CHO-K1 (b) and HCjE (c) using an insoluble substrate, X-gal, to monitor individual cells turning blue because of virus entry (shown in black). (B) Analysis of HSV-1(KOS) rid1 mutant entry into HCjE cells. HCjE or the control CHO-K1 cells were plated in 96-well culture dishes and inoculated by serial dilutions of β-galactosidase–expressing recombinant HSV-1(KOS)Rid1tk12 virus at the PFU/cell indicated. ONPG (a) and X-gal (b, c) entry assays were repeated, as described.