FIGURE 4.
Lysosomotropic agents negatively affect HSV-1 entry. Monolayers of HCjE cells were either mock treated (No Treatment) or treated with indicated concentrations of bafilomycin (a) or chloroquine (b) and exposed to HSV-1 (50 PFU/cell). HeLa cells were also subjected to the same treatments and served as the positive control. Viral entry was measured using a spectrophotometer 6 hours after infection. Data shown are the means of triplicate measures and are representative of three independent experiments. The control (No Treatment) represents entry into corresponding mock-treated cells.