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. 1997 Nov;8(11):2157–2169. doi: 10.1091/mbc.8.11.2157

Figure 4.

Figure 4

Addition of Fm MAP kinase can delay the onset of mitosis in activated CSF-arrested extracts. Extracts were supplemented with buffer (□), 0.4 μM Froggymaker MAP kinase (Fm, •), or 0.8 μM Fm (○) and incubated for 10 min before activation with 0.4 mM CaCl2. Aliquots were removed every 20 min for analysis. (A) Histone H1 kinase activity. (B) MAP kinase activity, assessed by an immune complex kinase assay with antiserum X15.