Figure 6.

Gene-target optimization for amplification of GAPDH and HPRT mRNA from fresh, FF, and FFPE tissue RNA isolates. Real-time quantitative RT-PCR was performed in three different target regions [5′ and middle region of open reading frame (ORF) and 3′ untranslational area of target gene] with gene-specific probe sets (see Supplemental Table 1), designed by Primer Express software (Applied Biosystems). The x-axis shows the region that is amplified for the target gene. The y-axis shows expression values for targeted RNA regions (log scale). Fresh rat kidney tissue RNA was used as a high-quality RNA control, and relative expression levels of each sample are normalized to the fresh tissue RNA. Data are from three independent experiments and are expressed as mean ± SD.