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Evaluation of the influence of normalization. Raw (unnormalized) data was used for comparison of differential metabolites between normal colon and colon carcinoma. Of the 24 highly significant metabolites 14 (58%) could be confirmed at the high (p < 0.00024, **) and 19 (79%) at the normal (p < 0.05, *) significance level, indicating that the major metabolite differences are not dependent on the normalization strategy.
