Fig. 1.

Microbiota-mediated increase in adiposity is blunted in Bt/Ms cocolonized gnotobiotic Gpr41−/− mice. (A) Weights of both epididymal fat pads in Gpr41−/− and +/+ littermates that are GF, or raised GF and then colonized at 4 to 6 weeks of age with Bt and Ms (n, 8–14 males per group; three independent experiments). (B) Weight gain (n, 4–9 mice per group; followed one to two times per week for up to 4 weeks, between 5 and 9 weeks of age in the case of GF animals, and for 4 weeks after gavage in the case of Bt/Ms cocolonized gnotobiotic animals). Mean values ± SEM are plotted. *, P < 0.05, **, P < 0.01. N.S., not significantly different.