Figure 7.

BFA inhibits constitutive GAG chain secretion from AtT-20 cells. Cells were treated with xyloside for 30 min and then pulse-chased with [³⁵S]sulfate for 5 min. BFA was added immediately after the pulse labeling. The cells were allowed to chase for up to 1 h. The amount of labeled GAG secreted into the medium was determined for each time point, and plotted as the percentage of total label recovered in the cell plus the medium for that time point. □, Secretion from control untreated cells; ○, secretion from BFA-treated cells.