Expression profile of EphA4 receptor after spinal cord injury. A single band of 120 kDa was detected (top panel) from spinal cord of naïve animals, and the specificity of the antibody determined by preabsorbing it with sc-921P blocking peptide. Representative immunoblots showed EphA4 expression at the lesion epicenter (T10 segment) at 2, 4, 7, 14 or 28 days post-injury from sham and injured animals. β-actin levels was used as a housekeeping protein that remained constant. Densitometric analysis of EphA4 immunoreactivity from sham and injured rats, expressed as percentage values relative to control levels.