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. Author manuscript; available in PMC: 2008 Oct 20.
Published in final edited form as: J Neurochem. 2005 Feb;92(4):824–830. doi: 10.1111/j.1471-4159.2004.02915.x

Fig. 4.

Fig. 4

Consequences of proteasome inhibition in HT22 cells. (a) In the presence of lactacystin, ubiquitinated high molecular mass proteins accumulate in the Triton-X-100-insoluble fraction. (b) Ubiquitinated proteins accumulate specifically in the mitochondrial fraction of lactacystin-treated HT22 cells. (c) After inhibition with lactacystin, oxygenated proteins also accumulate specifically in the mitochondrial fraction. Mitochondrial proteins were derivatized with dinitrophenylhydrazine (+ DNPH) or sham-treated (– DNPH).