Figure 8.

Immunoblot analysis of ciliary protein fractions from control and colchicine-treated late-gastrula stage embryos using the monoclonal antibody 7.10 to molecular chaperones of the HSP70 family shows that the localization of a 78-kDa cognate to HSP70 is minimally affected by colchicine treatment, but its total amount is greater. The antibody also cross-reacted with a 46-kDa axonemal protein. The original unfractionated axonemes that yielded these samples are shown in Figure 6, lanes 2 and 3, respectively.