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. Author manuscript; available in PMC: 2008 Oct 20.
Published in final edited form as: Mol Cell Neurosci. 2006 Dec 26;34(2):271–279. doi: 10.1016/j.mcn.2006.11.005

Figure 6. Astrocyte conditioned media induces motor neuron death in culture.

Figure 6

a) Conditioned media from stimulated astrocyte cultures (PN: peroxynitrite; SIN: SIN1; dPN: degraded PN) were used to prepare motor neuron media. The media were supplemented with neurotrophic factors immediately prior to use. Survival of motor neurons was quantified 48 hours after application of the astrocyte-conditioned MN media. b) Prior to survival experiments, astrocyte-conditioned MN media were immuno-depleted using an antibody against the pro-domain of proNGF. c) To confirm that the reduction in MN survival was due to the activation of sortilin, neurotensin was added to the neuronal cultures.