Figure 7. Non-homologous end-joining DNA repair assay.
Nuclear extracts were prepared from uninfected astrocytes and from astrocytes infected with Mad-1/SVEdelta JCV for 5, 11 and 14 days. NHEJ assays were performed with a linearized plasmid substrate as described in Materials and Methods and the reactions run on an ethidium bromide/agarose gel. The position of the products are indicated by the bracket. The substrate is indicated with an arrow. The lane labeled “*” indicates the negative control (no nuclear extract).