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. 2008 Nov;173(5):1311–1325. doi: 10.2353/ajpath.2008.080272

Figure 3.

Figure 3

PAI-1 overexpression stimulates accumulation of newly synthesized collagen in normal fibroblasts infected with adenoviral vector carrying the wild-type PAI-1 gene. Normal fibroblasts from the N144 strain were infected with empty vector (control) and Wt PAI-1 adenovirus at 30, 100, or 300 moi for 24 hours in 0.1% ITS+ before culturing in 1% FBS for 2, 6, or 13 days or in 10% FBS for 13 days. A: Western blots of PAI-1 protein expression (cell layer) under 1% FBS use the nuclear envelope proteins Lamin A/C as loading controls and represent the physical pool of triplicate samples. B: Analyses of accumulation of newly synthesized collagen use DNA content for normalization and represent the separate analysis of triplicate samples (□, empty vector; ░⃞, Wt PAI-1 vector; ▪, Wt PAI-1 minus Empty vector). The results represent the means ± SD. PAI-1 = 47 kDa; Lamin A/C = 74/65 kDa.