Figure 7.

PAI-1 protein expression and collagen accumulation in N144 normal fibroblasts infected with adenoviral vectors carrying wild-type PAI-1 or mutant genes. The protease inhibitor mutant (PAI-1^INH−), vitronectin-binding mutant (PAI-1^VN−), or Wt PAI-1 adenoviral vectors at 30,100, and 300 moi were used to infect cells for 24 hours in 0.1% ITS+ before 6 days of culture in 3% FBS. A: PAI-1 protein expression was analyzed by Western blotting with α-tubulin (52 kDa) as the loading control. Empty vector was used as the control. Each protein band represents a physical pool of triplicates samples. Light spots inside the PAI-1 protein bands in lanes 12 and 13 indicate local exhaustion of chemiluminescent substrate. B: Accumulation of newly synthesized collagen as analyzed in Figure 5. Keloid strain K-C3 was used for comparison with normal strain N144 and N144 transduced with empty vector or viruses expressing wild-type and mutant PAI-1 at 100 moi during infection and culture as in A. n = 3, *P ≤ 0.05; **P ≤ 0.01; ^#P = 0.13.