Figure 8.

PAI-1-specific protease inhibitory activity of PAI-1 protein from normal fibroblasts (N144) infected with adenoviral vectors expressing Wt PAI-1, PAI-1^INH−, or PAI-1^VN−. A: Fibroblasts were infected with three concentrations of adenoviruses, 30, 100, or 300 moi for 24 hours in 0.1% ITS+ before culturing for 6 days in 3% FBS. The activity of PAI-1 protein from culture media was analyzed using Spectrolyze (pL) PAI-1 assay (American Diagnostica). Tubulin levels in the cell layer were determined by Western blotting and image analysis and used to normalize the data for protease inhibition. n = 3; mean ± SD. B: Phase contrast images from cultures described above. Note morphological similarity, except for cells infected with Ad-PAI-1^VN−, which display some cell rounding. Scale bar = 15 μm.