Figure 1.

STAT1 activation by poly I:C treatment in M-SMCs. Electrophoretic mobility shift assays using labeled hSIE as a probe with equal amounts of whole cell extracts (15 μg) prepared from cells in the presence of 2% fetal bovine serum as indicated in figures. a: Activation of STAT protein in 2- or 4-hour poly I:C-treated and 30-minute IL-6-treated extracts. b: Supershift analyses with 4-hour poly I:C-treated extract or with 30-minute IL-6-treated extract were performed after pre-incubation with STAT1- or STAT3-specific antibody. c: Cells treated with and without poly I:C and cycloheximide 30 minutes before the poly I:C treatment. d: Cultured M-SMCs were treated for 4 hours with medium obtained from 4-hour poly I:C-treated M-SMCs. Several exposures were taken from the autoradiogram and one of the exposures was used for this figure.