Figure 6.

ATA Inhibit Parasitemia and Mortality of BALB/c Mice Infected with T. cruzi. A and B: Groups of five BALB/c mice were primed in the footpads with 6 ng of ATA (#1) or control non-chagasic IgG and, after 60 minutes, inoculated in the sensitizing footpad with 5 × 10³ trypomastigotes (Tulahuen strain). Mice were assessed for parasitemia (A) at the indicated times and observed daily for survival (B) and clinical symptoms. Experiment performed twice; results are plotted as average of the two experiments. C and D: Groups of five BALB/c mice were inoculated each in the footpad with T. cruzi (5 × 10³ trypomastigotes) and, 2 days later, injected intraperitoneally with 2.6 ng of control non-chagasic IgG or ATA (#1), and every second or third day up to 12 days, for a total of 250 ng autoantibody. Parasitemia (C) and survival (D) were assessed at the indicated times. *, P < 0.05; **, P < 0.001.