Table 1.
Primary Antibody and Antibody/Fluorochrome Conjugates against Various Immune Mediators, B. burgdorferi, and Phenotypic Markers of Cells
| Mediator/cell type | Primary Ab | Isotype | Dilution/ concentration | Source |
|---|---|---|---|---|
| IL-1β | Anti-human IL-1β | Rabbit IgG | 20 μg/ml | RDI, Flanders, NJ |
| IL-6 | Anti-human IL-6 | Mouse IgG2a | 5 μg/ml | Chemicon, Temecula, CA |
| IL-8 | Anti-human IL-8 | Rabbit IgG | 10 μg/ml | RDI |
| TNF-α | Anti-human TNF-α | Mouse IgG1 | 20 μg/ml | BD Pharmingen, San Diego, CA |
| Anti-rhesus macaque | Rat IgG1 | 20 μg/ml | R&D Systems, Minneapolis, MN | |
| TNF-α | ||||
| CXCL13 | Anti-human CXCL13 | Goat IgG | 5 μg/ml | R&D |
| COX-2 | Anti-human COX-2 | Rabbit IgG | 10 μg/ml | Chemicon |
| Activated caspase-3 | Anti-human | Rabbit IgG | 5 μg/ml | Abcam, Inc. |
| Active caspase-3 | Cambridge, MA | |||
| Ab clone 13847 | ||||
| Astrocyte | Anti-human | Mouse IgG1 | 1:200 | Sigma, St Louis, MO |
| GFAP-cy3 | ||||
| Anti-human GFAP | Mouse IgG1 | 1:200 | Sigma | |
| Microglia | Anti-Iba1 synthetic | Rabbit IgG | 1:100 | Wako Pure Chemicals, Richmond, VA |
| Oligodendrocyte | Anti-human S-100 | Rabbit IgG | 1:500 | Sigma |
| Anti-human MAB 328 | Mouse IgM | 1:200 | Chemicon | |
| Neuron | Anti-mouse NeuN | Mouse IgG1 | 1:20 | Chemicon |
| B. burgdorferi | Anti-whole cell preparation | Rabbit IgG | 1:250 | Accurate Chemicals, Westbury, NY |
| Anti-Bb-FITC | Goat | 1:10 | Kirkegaard and Perry, Gaithersburg, MD |