Figure 6.

Adoptive transfer of tolerance. Total lymphocytes harvested from popliteal LNs of tolerized and nontolerized animals were cultured in the presence of MAA for 3 days. Nonadherent cells (2 × 10⁷) were adoptively transferred to naïve Lewis rats and the animals were immunized with MAA 24 hours after the cell transfer. A: EAAU developed in two phases in Lewis rats that received LN cells from nontolerized rats whereas adoptive transfer of mixed lymphocytes from tolerized Lewis rats induced tolerance in the recipient rats. B: No inflammation could be seen at day 19 in the animals that received adoptively transferred mixed lymphocytes from tolerized animals. C: Ocular histopathology at day 19 after immunization revealed extremely severe (clinical score 6) EAAU in the animals that received total lymphocytes from the LNs of nontolerized animals. I, Iris; CB, ciliary body; AC, anterior chamber. D: Effect of CD4⁺CD25⁺ T-cell depletion on the adoptive transfer of tolerance. Adoptive transfer of lymphocytes harvested from popliteal LNs transferred tolerance to the recipient Lewis rats. Depletion of CD4⁺CD25⁺ T cell from the total lymphocytes harvested from popliteal LNs of tolerized animals impaired the ability of these cells to transfer tolerance. E: Effect of recombinant IL-2 on adoptive transfer of tolerance. Presence or absence of recombinant IL-2 in culture of total lymphocytes harvested from the popliteal LNs did not affect their ability to transfer tolerance to naïve rats. Total lymphocytes from nontolerized Lewis rats were used as control. Original magnifications, ×10.