Table 1.

Primer Sequences Used in RT-PCR for Each Protein

Gene	Primer	Sequence	Amplified PCR product size (bp)
β-Actin	Forward	5′-GCGCTCGTCGTCGACAACGG-3′	335
	Reverse	5′-GTGTGGTGCCAAATCTTCTCC-3′
IFN-γ	Forward	5′-ATCTGGAGGAACTGGCAAAAGGACG-3′	288
	Reverse	5′-CCTTAGGCTAGATTCTGGTGACAGC-3′
TNF-α	Forward	5′-ATGATCCGAGATGTGGAACTGGCA-3′	295
	Reverse	5′-GCTCCTCTGCTTGGTGGTTTGCTA-3′
IL-10	Forward	5′-AAGGACCAGCTGGACAACAT-3′	292
	Reverse	5′-AGACACCTTTGTCTTGGAGCTTA-3′
TGF-β2	Forward	5′-ATCGTCCGCTTCGATGTCTCAACA-3′	274
	Reverse	5′-ATCCCAGGTTCCTGTCTTTGTGGT-3′
IL-2	Forward	5′-TTGCACTGACGCTTGTCCTCCTT-3′	398
	Reverse	5′-CCATCTCCTCAGAAATTCCACCAC-3′

The sequences of all oligonucleotides are shown in 5′ to 3′ direction.