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. 2008 Nov;173(5):1464–1475. doi: 10.2353/ajpath.2008.071142

Figure 4.

Figure 4

Histology of skin abnormalities in Hai-1/Spint1-deleted mice at 7th day after birth. A: Expression of Hai-1/Spint1 and matriptase in the skin. Hai-1/Spint1 mRNA was undetectable in B6Hai-1−/−High skin derived from chimera generated by injection of B6Hai-1−/− ES 41 cells into Hai-1/Spint1+/+ ICR blastocysts even with sensitive RT-PCR study (35 cycles of amplification). In contrast, wild-type skin tissue shows Hai-1/Spint1 expression. Matriptase expression was equally observed in both groups. Results from two mice were shown in each group. B: Histology of the dorsal skin (H&E stain). B6Hai-1−/−High epidermis shows hypergranulosis with thick stratum corneum. Note that B6Hai-1−/− ES 1 and B6Hai-1−/− ES 41 resulted in the same epidermal abnormalities in high-chimerism mice. C: Histology of skin abnormalities at 14th day after birth. In B6Hai-1−/−High mouse, the external surface of the dorsal skin was covered by a thick desquamating keratin layer, and the epidermis showed acanthosis with mildly enlarged keratinocyte nuclei compared with that of low-chimerism mice or high-chimerism mice generated by B6Hai-1+/− (hetero) ES cells and ICR blastocysts (B6Hai-1+/−High Hetero). H&E stain. Scale bars = 100 μm.