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. 2008 Nov;173(5):1464–1475. doi: 10.2353/ajpath.2008.071142

Figure 9.

Figure 9

Enhanced phosphorylation of Akt in Hai-1/Spint1-deficient keratinocytes. A: Immunoblot analyses for phosphorylated Akt (p-Akt) of cultured keratinocytes. The same blot was reprobed with antibody against total Akt. The band intensity was measured and the ratio of p-Akt to the corresponding total Akt was calculated. The bar graph indicates means ± SD of p-Akt/total Akt ratio (n = 4). *P < 0.05, Mann-Whitney U-test. The results of PCR genotyping of the keratinocytes are also shown. B: Immunohistochemistry for p-Akt. Enhanced immunoreactivity of p-Akt was observed in Hai-1/Spint1-deficient epidermal keratinocytes. C: Ki-67 immunoreactivity of the epidermis and hair follicles. Scale bars = 100 μm.