Figure 3.
Quantitation of Rab27A in Muscle (A) shows immunoblot for Rab27A (∼55kDa) in patient muscle biopsies. Low levels of Rab27A protein is seen in normal control muscle (N1 and N2) and in FKRP dystrophy muscle (LGMD2I) (F1, F2 and F3) muscle, whereas dysferlin deficient patient muscle (D1, D2, D3 and D4) shows increased Rab27A protein. Rab27A protein expression levels were calculated as ratios relative to two control proteins; myosin heavy chain Coomassie Blue staining (B), and β-tubulin immunoblot (C).