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Map of the wild-type AAV-2 genome. (A) Rep and Cap genes flanked by ITRs. The different Rep and Cap transcripts are produced from their respective promoters (P5, P19, and P40). The star indicates the alternative ACG codon used to produce VP3. (B) Secondary structure of an AAV-2 ITR showing the RBEs (RBE, GAGCGAGCGAGCGCGC; RBE′, CTTG) and the TRS (GTTGG).
